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| Sartorello,R.; Garcia,C.R.S.. |
| An increasing number of pathophysiological roles for purinoceptors are emerging, some of which have therapeutic potential. Erythrocytes are an important source of purines, which can be released under physiological and physiopathological conditions, acting on purinergic receptors associated with the same cell or with neighboring cells. Few studies have been conducted on lizards, and have been limited to ATP agonist itself. We have previously shown that the red blood cells (RBCs) of the lizard Ameiva ameiva store Ca2+ in the endoplasmic reticulum (ER) and that the purinergic agonist ATP triggers a rapid and transient increase of [Ca2+]c by mobilization of the cation from internal stores. We also reported the ability of the second messenger IP3 to discharge... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Red blood cells; Calcium; Teiidae; Ameiva ameiva; Purinoceptor; UTP. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000100002 |
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| Costa,Maria Regina de Macedo; Ribeiro,Camila Godinho; Santos-Filho,Sebastião David; Neves,Rosane de Figueiredo; Fonseca,Adenilson de Souza da; Bernardo-Filho,Mario; Catanho,Maria Teresa Jansem de Almeida. |
| The development of experimental assays to study properties of herbal medicine is worthwhile. Vitex agnus castus (VAC) is utilized in popular medicine and some actions have been attributed to its extract. Blood cells (BC) and plasma proteins are labeled with technetium-99m (Tc-99m) and have been used in nuclear medicine, as in basic research. This procedure uses a reducing agent and stannous ion is utilized. There are reports that drugs can alter this labeling process. The aim of this work was to evaluate the influence of an aqueous extract of VAC on the labeling of blood constituents with Tc-99m. Blood was incubated with VAC, stannous chloride and Tc-99m, as sodium pertechnetate, and centrifuged. Samples of BC and plasma were separated, aliquots of BC and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Oxidant properties; Plasma proteins; Red blood cells; Technetium-99m; Vitex agnus-castus. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000600023 |
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| GUTIÉRREZ-CERVANTES,Adrián; AHUJA-AGUIRRE,Concepción; LÓPEZ-de BUEN,Lorena; VIVEROS-PEREDO,Sergio. |
| ABSTRACT The objective of the study was to determine the morphological characteristics of peripheral blood cells (erythrocytes, leukocytes, thrombocytes) and the leukocyte differential count (heterophils, eosinophils, basophils, lymphocytes, monocytes, azurophils) of captive Morelet's crocodiles (Crocodylus moreletii) from Veracruz, Mexico. Peripheral blood from 80 apparently healthy farmed crocodiles (39 subadults [19 females, 20 males] and 41 adults [18 females, 23 males]) was examined for morphology through stained blood smears and manual count was used for the leukocyte differential. Blood was collected during the non-breeding (n = 42) and breeding (n = 38) seasons. Blood examination indicated similar morphological characteristics of blood cells in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Crocodilians; Hematology; Red blood cells; White blood cells. |
| Ano: 2022 |
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2022000100017 |
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| Red blood cells (RBC) are viable if kept in an adequate preservative solution, although gradual changes in morphology and metabolism may occur. There is a gradual decrease in adenosine-5'-triphosphate (ATP) concentration, pH, glucose consumption, and enzyme activity during preservation. The normal discocyte shapes are initially replaced by echinocytes and stomatocytes and, at final stages, by spherocytes, the last step before splenic sequestration. Post-transfusional survival has been correlated with the ATP concentration. RBC preserved in ADSOL, a solution containing adenine, dextrose, sodium chloride, and mannitol, are viable for transfusion for up to 6 weeks. Erythrocytes from 10 blood units taken from healthy adult donors were preserved for 12 weeks in... |
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Palavras-chave: Red blood cells; Adenosine-5'-triphosphate; Blood transfusion. |
| Ano: 1997 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000600007 |
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| Paoli,Severo de; Giani,Tania Santos; Presta,Giuseppe Antonio; Pereira,Marcia Oliveira; Fonseca,Adenilson de Souza da; Brandão-Neto,José; Medeiros,Aldo da Cunha; Santos-Filho,Sebastião David; Bernardo-Filho,Mário. |
| Clove (Caryophyllus aromaticus L.) has been used for clinical procedures. Blood constituents labeled with technetium-99m (99mTc) are used in nuclear medicine. The aim of this work was to evaluate the effects of clove extract on the labeling blood constituents with 99mTc and on the morphology of red blood cells. Blood samples were incubated with clove, stannous chloride and 99mTc. Plasma, blood cells, insoluble fractions of plasma and blood cells were separated. The radioactivity was counted and percentage of radioactivity (%ATI) to each blood fraction was calculated. The shape and morphometric parameter (perimeter/area ratio) were evaluated. Clove extract altered significantly (p<0.05) the %ATI of blood constituents and the shape of red blood cells... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Technetium-99m; Blood constituents; Red blood cells; Morphology; Caryophyllus aromaticus L. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000600022 |
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| Leite,A.A.; Barretto,O.C. de O.. |
| Glucose-6-phosphate dehydrogenase (G6PD) activity and the affinity for its substrate glucose-6-phosphate were investigated under conditions similar to the physiological environment in terms of ionic strength (I: 0.188), cation concentration, pH 7.34, and temperature (37oC). A 12.4, 10.4 and 21.4% decrease was observed in G6PD B, G6PD A+ and G6PD A- activities, respectively. A Km increase of 95.1, 94.4 and 95.4% was observed in G6PD B, G6PD A+ and G6PD A-, respectively, leading to a marked decrease in affinity. In conclusion, the observation of the reduced activity and affinity for its natural substrate reflects the actual pentose pathway rate. It also suggests a much lower NADPH generation, which is crucial mostly in G6PD-deficient individuals, whose NADPH... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Glucose-6-phosphate dehydrogenase; Red blood cells; G6PD activity and affinity. |
| Ano: 1998 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001200004 |
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